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Capillary electrophoresis of proteins for proteomic studies
Author(s) -
Manabe Takashi
Publication year - 1999
Publication title -
electrophoresis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.666
H-Index - 158
eISSN - 1522-2683
pISSN - 0173-0835
DOI - 10.1002/(sici)1522-2683(19991001)20:15/16<3116::aid-elps3116>3.0.co;2-0
Subject(s) - capillary electrophoresis , isoelectric focusing , chromatography , chemistry , proteome , gel electrophoresis , proteomics , electrophoresis , gel electrophoresis of proteins , free flow electrophoresis , sodium dodecyl sulfate , mass spectrometry , polyacrylamide gel electrophoresis , biochemistry , enzyme , gene
Analyses of proteins in complex mixtures such as cell lyzates are presently performed mainly by sodium dodecyl sulfate (SDS)‐polyacrylamide gel electrophoresis. For structural analysis, each protein in a spot is digested with proteases and the fragment peptides are subjected to Edman sequencing and/or mass spectrometry. These works aim at the total analysis of proteins in a complex mixture and reconstruction of their cooperative functions. Genomic studies are now being combined with these proteomic studies. This review article focuses on the application of capillary electrophoresis aiming at the total analysis of complex protein systems or structural analysis of each separated protein. From this viewpoint, articles on capillary zone electrophoresis, capillary isoelectric focusing, and sieving SDS capillary electrophoresis are reviewed. Since these techniques of capillary electrophoresis have been thoroughly reviewed previously, papers published in 1997 and 1998 are mainly covered.