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Capillary electrophoresis of peptides
Author(s) -
Kašička Václav
Publication year - 1999
Publication title -
electrophoresis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.666
H-Index - 158
eISSN - 1522-2683
pISSN - 0173-0835
DOI - 10.1002/(sici)1522-2683(19991001)20:15/16<3084::aid-elps3084>3.0.co;2-4
Subject(s) - isotachophoresis , capillary electrophoresis , chromatography , isoelectric focusing , capillary electrochromatography , electrochromatography , chemistry , electrophoresis , mass spectrometry , micellar electrokinetic chromatography , electrokinetic phenomena , peptide , capillary electrophoresis–mass spectrometry , affinity electrophoresis , electrospray ionization , electrolyte , affinity chromatography , biochemistry , electrode , enzyme
This article gives a review of the recent developments in capillary electrophoresis (CE) of peptides. New approaches to the theoretical description of electromigration behavior of peptides are described, and methodological aspects of CE separations of peptides such as selection of separation conditions, sample treatment, suppression of peptide adsorption to the capillary wall and specificities of CE separation modes are discussed. Progress in application of high performance detection schemes, namely laser‐induced fluorescence and mass spectrometry, in peptide separations by CE is presented. Applications of different CE techniques, zone electrophoresis, isotachophoresis, isoelectric focusing, affinity electrophoresis, electrokinetic chromatography and electrochromatography to peptide analysis, preparation and physicochemical characterization are demonstrated.