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Recent developments in DNA sequencing by capillary and microdevice electrophoresis
Author(s) -
Schmalzing Dieter,
Koutny Lance,
SalasSolano Oscar,
Adourian Aram,
Matsudaira Paul,
Ehrlich Daniel
Publication year - 1999
Publication title -
electrophoresis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.666
H-Index - 158
eISSN - 1522-2683
pISSN - 0173-0835
DOI - 10.1002/(sici)1522-2683(19991001)20:15/16<3066::aid-elps3066>3.0.co;2-x
Subject(s) - capillary electrophoresis , dna sequencer , dna sequencing , oligonucleotide , electrophoresis , genotyping , dna , gel electrophoresis , massive parallel sequencing , gel electrophoresis of nucleic acids , chromatography , biology , computational biology , chemistry , microbiology and biotechnology , genetics , gene , genotype
Abstract The present review covers papers published in the years 1997 and 1998 on DNA sequencing by capillary and microdevice electrophoresis. The article does not include other electrophoretic DNA applications such as analysis of oligonucleotides, genotyping, and mutational analysis. Capillary gel electrophoresis (CGE) is starting to become a viable competitor to slab gel electrophoresis for DNA sequencing. Commercially available multicapillary array sequencers are now entering sequencing facilities which to date have totally relied on traditional slab gel technology. CGE research on DNA sequencing therefore becomes increasingly concerned with the critical task of fine‐tuning the operational parameters to create robust sequencing systems. Electrophoretic microdevices are being considered the next technological step in DNA sequencing by electrophoresis.