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DNA typing of fingerprints using capillary electrophoresis: Effect of dactyloscopic powders
Author(s) -
Van Hoofstat David E. O.,
Deforce Dieter L. D.,
Hubert De Pauw Isabel P.,
Van den Eeckhout Elfride G.
Publication year - 1999
Publication title -
electrophoresis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.666
H-Index - 158
eISSN - 1522-2683
pISSN - 0173-0835
DOI - 10.1002/(sici)1522-2683(19991001)20:14<2870::aid-elps2870>3.0.co;2-v
Subject(s) - capillary electrophoresis , typing , chromatography , dna , dna profiling , chemistry , electrophoresis , fingerprint (computing) , microbiology and biotechnology , biology , genetics , computer science , artificial intelligence
DNA typing is a useful tool in crime solving, not only for blood samples, sperm, or sa liva but also for traces of DNA left on tools or pieces of clothing used in burglaries or thefts. On these kinds of samples, the sources of DNA are extremely small amounts of skin debris left after gripping tools. When a sensitive technique such as polymerase chain reaction (PCR) coupled with capillary electrophoresis is used, it is possible to get a profile from these low amounts of DNA. The classic technique in such cases, used in forensic sciences, is to reveal fingerprints by different dactyloscopic powders. Therefore, DNA profiling was performed on physical fingerprints left on glass and wooden plates, in order to establish eventual problems or interferences involved by using both techniques simultaneously. Eleven dactyloscopic powders were investigated on their influence on DNA typing. The results show that some can be used together with DNA profiling but that serious precautions have to be taken to avoid contamination.