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Genotyping Taenia tapeworms by single‐strand conformation polymorphism of mitochondrial DNA
Author(s) -
Gasser Robin B.,
Zhu Xingquan,
Woods Wayne
Publication year - 1999
Publication title -
electrophoresis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.666
H-Index - 158
eISSN - 1522-2683
pISSN - 0173-0835
DOI - 10.1002/(sici)1522-2683(19991001)20:14<2834::aid-elps2834>3.0.co;2-f
Subject(s) - single strand conformation polymorphism , taenia , biology , mitochondrial dna , polymerase chain reaction , genetics , genomic dna , cytochrome c oxidase , dna , nadh dehydrogenase , microbiology and biotechnology , gene , mitochondrion , zoology , helminths
To overcome limitations in identifying tapeworms of the genus Taenia by traditional approaches, we have established a single‐strand conformation polymorphism (SSCP) method utilizing two different regions of mitochondrial (mt) DNA as targets. The NADH dehydrogenase 1 and the cytochrome c oxidase subunit I genes were amplified from genomic DNA by polymerase chain reaction (PCR), denatured and subjected to electrophoresis in mutation detection enhancement gels. SSCP analysis achieved delineation among eight different species of Taenia from different hosts based on characteristic profiles and enabled the detection of intraspecific variability in profiles for some taxa. This SSCP‐based typing method has important implications for taxonomy, diagnosis and for studying the genetic structure of Taenia populations.