Premium
Enantiomeric separations of dansyl amino acids using the macrocyclic antibiotic A35512B as a chiral selector in capillary electrophoresis
Author(s) -
Risley Donald S.,
TrelliSeifert Letithia,
McKenzie Quovadis J.
Publication year - 1999
Publication title -
electrophoresis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.666
H-Index - 158
eISSN - 1522-2683
pISSN - 0173-0835
DOI - 10.1002/(sici)1522-2683(19990901)20:13<2749::aid-elps2749>3.0.co;2-l
Subject(s) - capillary electrophoresis , enantiomer , chemistry , analyte , chromatography , amino acid , antibiotics , electrophoresis , selectivity , organic chemistry , biochemistry , catalysis
The macrocyclic antibiotic A35512B was examined as a chiral selector for capillary electrophoresis (CE) using thirteen racemic dansyl amino acids as test analytes. The chiral selectivity of A35512B was evaluated as a function of the run buffer pH, antibiotic concentration, and organic modifier composition. After optimizing these parameters, the macrocylic antibiotic A35512B provided high resolutions of all the enantiomers for the thirteen dansyl amino acids tested in this study.