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Alcohols and wide‐bore capillaries in nonaqueous capillary electrophoresis
Author(s) -
Porras Simo P.,
Jussila Matti,
Sinervo Kai,
Riekkola MarjaLiisa
Publication year - 1999
Publication title -
electrophoresis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.666
H-Index - 158
eISSN - 1522-2683
pISSN - 0173-0835
DOI - 10.1002/(sici)1522-2683(19990801)20:12<2510::aid-elps2510>3.0.co;2-l
Subject(s) - chemistry , electrolyte , capillary electrophoresis , acetic acid , acetonitrile , alcohol , chromatography , ammonium acetate , methanol , butanol , electrophoresis , propanol , ethanol , organic chemistry , high performance liquid chromatography , electrode
The feasibility of using C 1 ‐C 5 alcohols as electrolyte solutions in nonaqueous capillary zone electrophoresis was investigated. The separation of basic narcotic analgesics and acidic diuretics was modified by changing the alcohol in an electrolyte solution containing alcohol‐acetonitrile‐acetic acid (50:49:1, v/v) and 20 m M ammonium acetate while other experimental conditions were kept constant. The alcohols studied were methanol, ethanol, 1‐propanol, 2‐propanol, 1‐butanol, 2‐butanol, and 1‐pentanol. The results indicate that even longer‐chain alcohols can be used in nonaqueous capillary zone electrophoresis and, because of the lower currents they allow, they are especially advantageous in wider capillaries. Basic analytes were separated in 200 μm and 320 μm ID capillaries with 1‐butanol‐acetonitrile‐acetic acid (50:49:1, v/v) containing 20 m M ammonium acetate as electrolyte solution. Problems related to the use of wide‐bore capillaries are discussed.