Premium
Simultaneous analysis of lead, mercury and selenium species by capillary electrophoresis with combined ethylenediaminetetraacetic acid complexation and field‐amplified stacking injection
Author(s) -
Liu Wuping,
Lee Hian Kee
Publication year - 1999
Publication title -
electrophoresis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.666
H-Index - 158
eISSN - 1522-2683
pISSN - 0173-0835
DOI - 10.1002/(sici)1522-2683(19990801)20:12<2475::aid-elps2475>3.0.co;2-m
Subject(s) - capillary electrophoresis , ethylenediaminetetraacetic acid , chemistry , analyte , chromatography , detection limit , electrolyte , mercury (programming language) , selenium , stacking , electrophoresis , chelation , analytical chemistry (journal) , inorganic chemistry , organic chemistry , electrode , computer science , programming language
In this study, speciation analysis was carried out for multispecies of lead, mercury and selenium by capillary electrophoresis (CE) with combined complexation and field‐amplified stacking injection (FASI). Ethylenediaminetetraacetic acid (EDTA) was added to the sample mixture to form UV‐absorbing complexes with the analytes, thus enabling direct UV detection. It was also used in the running electrolyte to improve resolution. Under optimal conditions, ten analytes were baseline‐separated within 20 min. The influence of operational conditions (pH, EDTA concentration, sodium dodecyl sulfate concentration) on the electrophoretic behavior of the species was studied. The analyte‐related parameters were calculated based on the migration equation. Enhancement of detection, up to 1700‐fold, was achieved for the charged complexes with FASI, and sub‐ng/mL detection limits were obtained. The performance of this method was compared with high‐performance liquid chromatography, gas chromatography and other CE procedures.