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Analysis of spiramycin by capillary electrophoresis
Author(s) -
GonzálezHernández Rolando,
Li YongMin,
Van Schepdael Ann,
Roets Eugène,
Hoogmartens Jos
Publication year - 1999
Publication title -
electrophoresis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.666
H-Index - 158
eISSN - 1522-2683
pISSN - 0173-0835
DOI - 10.1002/(sici)1522-2683(19990801)20:12<2407::aid-elps2407>3.0.co;2-o
Subject(s) - spiramycin , capillary electrophoresis , chromatography , electrophoresis , chemistry , antibiotics , biochemistry , erythromycin
The development and validation of an analytical method for the determination of spiramycin I in the presence of its related substances by capillary electrophoresis is shown. The separation, performed in a phosphate buffer (80 m M , pH 7.5) containing 12 m M cetyltrimethylammonium bromide (CTAB) and 20 m M sodium cholate, with a 50 μm ID and 44 cm long fused‐silica capillary (36 cm effective length), applying a voltage of 12 kV (I ˜80 μA), at 25°C, is achieved in 15 min. Good selectivity among spiramycin I and its related substances was obtained. The influence of the buffer pH, and of the CTAB and sodium cholate concentrations was investigated. The method robustness, examined by means of a full‐fraction factorial design, shows that it can be used within the limits set for the three parameters that were investigated. The method is linear ( r = 0.9992) and precise (day‐to‐day corrected peak area repeatability, n = 18, relative standard deviation = 1.3%). The limits of detection and quantitation are 7 pg (0.025%) and 22 pg (0.08%), respectively, relative to a 2 mg/mL solution.