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Nonaqueous electrochromatography on C 30 columns: Separation of retinyl esters
Author(s) -
Roed Line,
Lundanes Elsa,
Greibrokk Tyge
Publication year - 1999
Publication title -
electrophoresis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.666
H-Index - 158
eISSN - 1522-2683
pISSN - 0173-0835
DOI - 10.1002/(sici)1522-2683(19990801)20:12<2373::aid-elps2373>3.0.co;2-3
Subject(s) - chromatography , chemistry , acetonitrile , retinyl palmitate , electrochromatography , dimethylformamide , methanol , selectivity , phase (matter) , elution , capillary electrochromatography , stationary phase , vitamin , organic chemistry , retinol , solvent , biochemistry , catalysis
A nonaqueous packed capillary electrochromatographic reversed‒phase method for separation of retinyl esters has been developed. The retinyl esters all‒ trans ‒retinyl acetate, palmitate, heptadecanoate, stearate, oleoate, and linoleoate were separated on a 180 μm ID column packed with 5 μm C 30 particles with a mobile phase consisting of 2.5 m M lithium acetate in N,N ‒dimethylformamide‒methanol (99:1, v/v). With this mobile phase, the electroosmotic flow was 0.8 mm/s at 650 V/cm and 40°C, and the separation was completed in less than 30 min on 30 cm columns. To obtain electrostable frits of the hydrophobic C 30 material both the preparation of the frits and the conditioning of the column prior to electroconditioning were of importance. Selectivity changes were introduced by replacing up to 70% v/v of the N,N ‒dimethylformamide by acetonitrile. The increase in the amount of acetonitrile was, however, accompanied by a significant increase in analysis time. Liver extracts obtained from arctic seal were analyzed.