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Two‐dimensional gel protein database of Saccharomyces cerevisiae (update 1999)
Author(s) -
Perrot Michel,
Sagliocco Francis,
Mini Thierry,
Monribot Christelle,
Schneider Ulrich,
Shevchenko Andrej,
Mann Mathias,
Jenö Paul,
Boucherie Hélian
Publication year - 1999
Publication title -
electrophoresis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.666
H-Index - 158
eISSN - 1522-2683
pISSN - 0173-0835
DOI - 10.1002/(sici)1522-2683(19990801)20:11<2280::aid-elps2280>3.0.co;2-q
Subject(s) - yeast , proteome , identification (biology) , spots , gel electrophoresis , saccharomyces cerevisiae , mass spectrometry , database , gene , biology , two dimensional gel electrophoresis , computational biology , biochemistry , proteomics , chemistry , chromatography , computer science , botany
By proving the opportunity to visualize several hundred proteins at a time, two‐dimensional (2‐D) gel electrophoresis is an important tool for proteome research. In order to take advantage of the full potential of this technique for yeast studies, we have undertaken a systematic identification of yeast proteins resolved by this technique. We report here the identification of 92 novel protein spots on the yeast 2‐D protein map. These identifications extend the number of protein spots identified on our yeast reference map to 401. These spots correspond to the products of 279 different genes. They have been essentially identified by three methods: gene overexpression, amino acid composition and mass spectrometry. These data can be accessed on the Yeast Protein Map server ( htpp://www.ibgc.u‐bordeaux2.fr/YPM ).