Premium
Micellar electrokinetic capillary chromatography of psoralen and isopsoralen
Author(s) -
Wang Dexian,
Yang Gengliang,
Engelhardt Heinz,
Zhang Hongyi
Publication year - 1999
Publication title -
electrophoresis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.666
H-Index - 158
eISSN - 1522-2683
pISSN - 0173-0835
DOI - 10.1002/(sici)1522-2683(19990701)20:9<1895::aid-elps1895>3.0.co;2-l
Subject(s) - micellar electrokinetic chromatography , psoralen , chemistry , chromatography , sodium dodecyl sulfate , psoralea corylifolia , capillary electrophoresis , medicine , dna , biochemistry , alternative medicine , pathology
In this paper, a micellar electrokinetic capillary chromatography (MEKC) method to analyze the active components of psoralen and isopsoralen in the Chinese herb Psoralea corylifolia L. was developed. It was found that the optimum separation condition for the analysis of these compounds was a buffer solution which contained phosphate, borate and sodium dodecyl sulfate (pH 9.2). Through optimization of the experimental condition, the two isomers could be baseline‐separated and their contents in the methanol‐extracted sample were quantitatively analyzed.