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Enantiomeric separation of amino acids by copper (II)‐ L ‐arginine ligand exchange capillary zone electrophoresis
Author(s) -
Yuan Zhuobin,
Yang Lili,
Zhang Shusheng
Publication year - 1999
Publication title -
electrophoresis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.666
H-Index - 158
eISSN - 1522-2683
pISSN - 0173-0835
DOI - 10.1002/(sici)1522-2683(19990701)20:9<1842::aid-elps1842>3.0.co;2-c
Subject(s) - capillary electrophoresis , chemistry , arginine , enantiomer , amino acid , chromatography , ligand (biochemistry) , molar ratio , resolution (logic) , electrophoresis , copper , stereochemistry , biochemistry , receptor , organic chemistry , catalysis , artificial intelligence , computer science
A novel method for the separation of amino acid enantiomers by ligand exchange capillary zone electrophoresis (CZE) using a chiral Cu(II)‐ L ‐arginine ligand was developed in this paper. The effects of the buffer concentration, pH, the molar ratio of L ‐arginine to Cu(II), and the total concentrations of Cu(II) and L ‐arginine on the migration times and resolutions of chiral amino acids were investigated. The optimum experimental conditions were 40 mmol/L of NH 4 OAc, pH 7.0, the 2:1 concentration ratio of L ‐arginine to Cu(II), 1 mmol/L CuSO 4 ·5H 2 O and 2 mmol/L L ‐arginine. Using the proposed method, six different dansyl‐amino acids were separated successfully with the resolution ranging from 1.1 to 2.3 in less than 15 min.