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Identification and localisation of ATP P 2X receptors in rat midbrain
Author(s) -
Worthington Rebecca A.,
Arumugam Thiruma V.,
Hansen Mitchell A.,
Balcar Vladimir J.,
Barden Julian A.
Publication year - 1999
Publication title -
electrophoresis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.666
H-Index - 158
eISSN - 1522-2683
pISSN - 0173-0835
DOI - 10.1002/(sici)1522-2683(19990701)20:10<2077::aid-elps2077>3.0.co;2-0
Subject(s) - identification (biology) , receptor , midbrain , chemistry , neuroscience , microbiology and biotechnology , computational biology , biophysics , biology , biochemistry , central nervous system , botany
P 2X receptors that are gated by extracellular ATP are among the few known examples of ligand‐gated cation‐selective channels. There have been seven cloned proteins identified to date as members of the P 2X receptor family in a wide range of tissues from the peripheral and central nervous systems and from many species. To determine the distribution of the P 2X subtypes in the rat midbrain, sodium dodecyl sulphate‐polyacrylamide gel electrophoresis (SDS‐PAGE)/Western blotting was combined with immunolocalisation using confocal microscopy. Subtypes P 2X1‐6 were detected in the periaqueductal gray area and the ependymal layer bordering the ventricle with a widespread distribution.