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Localisation of P 2X receptors in human salivary gland epithelial cells and human embryonic kidney cells by sodium dodecyl sulfate‐polyacrylamide gel electrophoresis/Western blotting and immunofluorescence
Author(s) -
Worthington Rebecca A.,
Dutton Julie L.,
Poronnik Philip,
Bennett Maxwell R.,
Barden Julian A.
Publication year - 1999
Publication title -
electrophoresis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.666
H-Index - 158
eISSN - 1522-2683
pISSN - 0173-0835
DOI - 10.1002/(sici)1522-2683(19990701)20:10<2065::aid-elps2065>3.0.co;2-e
Subject(s) - blot , embryonic stem cell , hek 293 cells , immunofluorescence , receptor , salivary gland , biology , microbiology and biotechnology , cell culture , kidney , cell , intracellular , endogeny , endocrinology , antibody , biochemistry , immunology , gene , genetics
Human salivary gland epithelial cells, a continuous cell line derived from an irradiated human salivary gland and human embryonic kidney cell line human embryonic kidney (HEK)293 were examined for the purpose of establishing whether they expressed endogenous P 2X ionotropic receptors at any stage in their cycles. HSG cells were found to express P 2X1—6 subtypes using both Western blotting and immunofluorescence labeling. HEK293 cells had no detectable levels of P 2X1—3 and P 2X6 under normal circumstances along with very low levels of P 2X4 and P 2X5 but when the cells were grown past confluence then all subtypes were expressed on the surface membrane with the exception of P 2X2 . The results are discussed in terms of the likely influence of ATP acting as an intercellular signaling molecule.