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On the mechanism of background silver staining during sodium dodecyl sulphate‐polyacrylamide gel electrophoresis
Author(s) -
Patras Georgia,
Qiao Greg G.,
Solomon David H.
Publication year - 1999
Publication title -
electrophoresis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.666
H-Index - 158
eISSN - 1522-2683
pISSN - 0173-0835
DOI - 10.1002/(sici)1522-2683(19990701)20:10<2039::aid-elps2039>3.0.co;2-x
Subject(s) - silver stain , polyacrylamide gel electrophoresis , chemistry , staining , gel electrophoresis , gel electrophoresis of proteins , sodium dodecyl sulfate , sodium , chromatography , electrophoresis , two dimensional gel electrophoresis , mechanism (biology) , polyacrylamide , biochemistry , microbiology and biotechnology , biology , gene , proteomics , genetics , enzyme , philosophy , epistemology , organic chemistry , polymer chemistry
A novel experimental procedure to study the factors that contribute to background silver staining in sodium dodecyl sulphate‐polyacrylamide gel electrophoresis (SDS‐PAGE) is described. A mechanism is proposed for background silver staining, which was found to result primarily from the redox initiator system using ammonium persulphate (APS) and an amine such as N,N,N′,N′ ‐tetramethylethylenediamine (TEMED).