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Separation of double‐stranded and single‐stranded DNA in polymer solutions: I. Mobility and separation mechanism
Author(s) -
Heller Christoph
Publication year - 1999
Publication title -
electrophoresis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.666
H-Index - 158
eISSN - 1522-2683
pISSN - 0173-0835
DOI - 10.1002/(sici)1522-2683(19990701)20:10<1962::aid-elps1962>3.0.co;2-h
Subject(s) - polymer , dna , capillary electrophoresis , electrophoresis , double stranded , scaling , chemical physics , separation (statistics) , matrix (chemical analysis) , separation method , chemistry , linear polymer , materials science , chromatography , computer science , organic chemistry , biochemistry , geometry , mathematics , machine learning
We have studied the separation of single‐stranded and double‐stranded DNA in a matrix of entangled, linear poly‐ N,N ‐dimethylacrylamide. Our results give better insight into the mechanisms involved during separations in polymer solutions. The dependence of different parameters on DNA size, electric field, pore size and the polymer chain length are evaluated and compared to theoretical predictions. Striking differences between experimental data and predicted scaling laws are found. Our data should help to optimize DNA separation in capillary electrophoresis and to improve existing models for DNA separation in porous matrices.