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Enantioseparation of tropa alkaloids by means of anionic cyclodextrin‐modified capillary electrophoresis
Author(s) -
Wedig Maike,
Holzgrabe Ulrike
Publication year - 1999
Publication title -
electrophoresis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.666
H-Index - 158
eISSN - 1522-2683
pISSN - 0173-0835
DOI - 10.1002/(sici)1522-2683(19990601)20:7<1555::aid-elps1555>3.0.co;2-f
Subject(s) - chemistry , enantiomer , capillary electrophoresis , cyclodextrin , sulfation , chromatography , atropine , beta cyclodextrins , beta (programming language) , scopolamine , stereochemistry , pharmacology , biochemistry , anesthesia , medicine , computer science , programming language
Anionic cyclodextrins (CDs), i.e. , sulfated CD, sulfobutylether β‐CD (SBE‐β‐CD) and heptakis(2,3‐di‐ O ‐acetyl‐6‐sulfato)‐β‐CD (HDAS‐β‐CD), were used to separate the isomers of atropine, homatropine, ipratropium, scopolamine and butylscopolamine. Variations in the pH value and the concentration of the various CDs revealed the sulfated β‐CD to be superior to the other derivatives. In a basic medium at low concentrations of sulfated CD the enantiomers of atropine, homatropine and ipratropium were well resolved in a short migration time, which is appropriate for the routine analysis of the enantiomeric excess. Scopolamine and butylscopolamine compounds could not be separated with either CD.