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High‐resolution electrophoretic analysis of rat parotid salivary proteins
Author(s) -
Williams Katherine M.,
Ekström Jörgen,
Marshall Thomas
Publication year - 1999
Publication title -
electrophoresis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.666
H-Index - 158
eISSN - 1522-2683
pISSN - 0173-0835
DOI - 10.1002/(sici)1522-2683(19990601)20:7<1373::aid-elps1373>3.0.co;2-z
Subject(s) - vasoactive intestinal peptide , silver stain , secretagogue , saliva , calcitonin gene related peptide , chemistry , staining , stimulation , coomassie brilliant blue , calcitonin , polyacrylamide gel electrophoresis , neuropeptide , endocrinology , medicine , biochemistry , microbiology and biotechnology , biology , receptor , enzyme , genetics
Silver staining and high‐resolution electrophoretic methods have been used to compare the protein composition of rat parotid saliva evoked in response to (i) parasympathetic stimulation (including the nonadrenergic, noncholinergic, atropine‐associated secretion), (ii) sympathetic stimulation, or (iii) the infusion of neuropeptides with secretagogue activity (substance P, calcitonin gene‐related peptide, neuropeptide Y, or vasoactive intestinal peptide). The different stimuli influenced the protein concentration and flow rate of the evoked secretion but had little effect upon the protein composition of the saliva. In contrast to earlier studies using sodium dodecyl sulfate‐polyacrylamide gel electrophoresis (SDS‐PAGE) and Coomassie blue staining, the combination of silver staining and two‐dimensional electrophoresis (2‐DE) revealed many newly detected proteins. The results indicate that the protein composition of rat parotid saliva is more complex than previously reported but is unaffected by the mode of stimulation.