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Low‐tech electrophoresis, small but beautiful, and effective: Electrophoretic titration curves of proteins
Author(s) -
Gianazza Elisabetta,
Miller Ingrid,
Eberini Ivano,
Castiglioni Silvia
Publication year - 1999
Publication title -
electrophoresis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.666
H-Index - 158
eISSN - 1522-2683
pISSN - 0173-0835
DOI - 10.1002/(sici)1522-2683(19990601)20:7<1325::aid-elps1325>3.0.co;2-x
Subject(s) - electrophoresis , titration , titration curve , chromatography , chemistry
Migration across a stationary pH gradient results in the electrophoretic titration of a protein′s dissociable groups. From the resulting curves, some properties of the protein may be derived, including overall amino acid composition and type of mutation between polymorphic variants, as well as range of stability or, for enzymes, of catalytic activity. Analysis with this technique is a stringent purity criterion; other applications allow the study of interacting systems and the planning of chromatographic fractionations based on differences in surface charge.