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Electrophoretic analysis of Bence Jones proteinuria
Author(s) -
Marshall Thomas,
Williams Katherine M.
Publication year - 1999
Publication title -
electrophoresis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.666
H-Index - 158
eISSN - 1522-2683
pISSN - 0173-0835
DOI - 10.1002/(sici)1522-2683(19990601)20:7<1307::aid-elps1307>3.0.co;2-p
Subject(s) - bence jones protein , capillary electrophoresis , electrophoresis , isoelectric focusing , chemistry , gel electrophoresis of proteins , chromatography , immunoelectrophoresis , immunofixation , paraproteins , sodium dodecyl sulfate , electropherogram , gel electrophoresis , polyacrylamide gel electrophoresis , immunoglobulin light chain , biochemistry , medicine , antibody , monoclonal , monoclonal antibody , immunology , enzyme
The electrophoresis of Bence Jones proteinuria (BJP) by urinary protein electrophoresis (UPE), immunoelectrophoresis (IE), immunofixation electrophoresis (IFE), sodium dodecyl sulfate‐polyacrylamide gel electrophoresis (SDS‐PAGE), isoelectric focusing (IEF), two‐dimensional electrophoresis (2‐DE) and capillary electrophoresis (CE) is described. UPE, IE and IFE are briefly discussed as clinical laboratory methods for the detection and typing of free light chain (LC) whilst the high resolution electrophoretic methods (SDS‐PAGE, IEF, 2‐DE and CE) are considered in greater detail as research tools for molecular characterisation of free LC and its association with nephrotoxicity. Refinements of sample processing designed to improve the standardisation of analysis of BJP by high resolution electrophoretic methods are reported.