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Two‐dimensional electrophoresis of Cereus peruvianus (Cactaceae) callus tissue proteins
Author(s) -
Mangolin Claudete A.,
Ottoboni Laura M. M.,
Machado M. de F. P. S.
Publication year - 1999
Publication title -
electrophoresis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.666
H-Index - 158
eISSN - 1522-2683
pISSN - 0173-0835
DOI - 10.1002/(sici)1522-2683(19990301)20:3<626::aid-elps626>3.0.co;2-f
Subject(s) - kinetin , callus , in vitro , biology , electrophoresis , tissue culture , chemistry , microbiology and biotechnology , biochemistry , botany
Two‐dimensional electrophoresis of Cereus peruvianus callus tissues grown in culture media containing two different 2,4‐dichlorophenoxyacetic acid (2,4‐D) and kinetin combinations was used to identify minor differences in polypeptide composition of these cell clones. Altered expression during growth in the two 2,4‐D and kinetin combinations was apparent for 13 polypeptides when calluses in the two media were compared. The number of proteins with differential expression (presence or absence of specific spots) was higher in callus tissues cultured in the 4.0 mg/L 2,4‐D and 8.0 mg/L kinetin combination than in callus tissues cultured in the 4.0 mg/L 2,4‐D and 4.0 mg/L kinetin combination. The present results show that the callus tissues maintained at 4.0 mg/L 2,4‐D and 8.0 mg/L kinetin can be used as a matrix for in vitro selection programs.