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Identification of squamous cell carcinoma antigen‐2 in tumor tissue by two‐dimensional electrophoresis
Author(s) -
Nawata Shugo,
Murakami Akihiro,
Hirabayashi Kei,
Sakaguchi Yuko,
Ogata Hidenobu,
Suminami Yoshinori,
Numa Fumitaka,
Nakamura Kazuyuki,
Kato Hiroshi
Publication year - 1999
Publication title -
electrophoresis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.666
H-Index - 158
eISSN - 1522-2683
pISSN - 0173-0835
DOI - 10.1002/(sici)1522-2683(19990301)20:3<614::aid-elps614>3.0.co;2-r
Subject(s) - microbiology and biotechnology , antigen , serpin , biology , gel electrophoresis , gene , biochemistry , immunology
The aim of this study was to identify two homologous serine proteinase inhibitor (serpin) molecules, squamous cell carcinoma (SCC) antigen‐1 and ‐2, by two‐dimensional electrophoresis (2‐DE), combined with immunoblotting, and examine their expression in tumor tissue. The recombinant SCC (rSCC) antigen‐1 showed four spots with p I 6.5, 6.4, 6.3 and 6.0, whereas rSCC antigen‐2 showed a more acidic spot with p I 5.95. SCC antigen in tumor tissue appeared in three new acidic spots (p I 5.7—5.5, M r 44 500), numbered 5, 6 and 7, besides the previously reported four spots numbered 1 to 4. These new acidic spots of SCC antigen apparently increased in SCC tissue. Treatment of tissue extract by carboxymethyl (CM)‐papain agarose matrix extinguished spots 1 to 4 encoded on the SCCA1 gene, but not 5 to 7 on the SCCA2 gene. Overexpression of the SCCA2 gene may play an important role in the malignant behavior of tumor cells.