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A capillary‐array electrophoresis system using side‐entry on‐column laser irradiation combined with glass rod lenses
Author(s) -
Anazawa Takashi,
Takahashi Satoshi,
Kambara Hideki
Publication year - 1999
Publication title -
electrophoresis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.666
H-Index - 158
eISSN - 1522-2683
pISSN - 0173-0835
DOI - 10.1002/(sici)1522-2683(19990301)20:3<539::aid-elps539>3.0.co;2-3
Subject(s) - capillary action , capillary electrophoresis , laser , irradiation , materials science , optics , throughput , chromatography , optoelectronics , chemistry , computer science , composite material , physics , nuclear physics , telecommunications , wireless
We have developed a simple and high‐throughput capillary‐array electrophoresis system that uses side‐entry on‐column laser irradiation. The number of capillaries in an array is generally limited by laser‐power attenuation along the array due to reflection and divergence. We overcame these problems by placing the capillaries in water and adding glass rod lenses between the capillaries. As a result, up to 45 capillaries could be simultaneously irradiated with a single laser beam and the fluorescence from all the capillaries could be detected with high sensitivity. We demonstrated the high throughput of 12 kbp/h with a 45 capillary array using this system.