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Fifty‐nine bp repeat polymorphism in the uncommon intron 36 of the human mucingene MUC5B
Author(s) -
Desseyn JeanLuc,
Rousseau Karine,
Laine Anne
Publication year - 1999
Publication title -
electrophoresis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.666
H-Index - 158
eISSN - 1522-2683
pISSN - 0173-0835
DOI - 10.1002/(sici)1522-2683(19990301)20:3<493::aid-elps493>3.0.co;2-7
Subject(s) - minisatellite , variable number tandem repeat , genetics , biology , locus (genetics) , intron , southern blot , tandem repeat , repeated sequence , polymerase chain reaction , allele , microsatellite , microbiology and biotechnology , exon , gene , genome
A variable number of tandem repeat (VNTR) polymorphism within the intron 36 of the human mucin gene MUC5B , which is mapped to chromosome 11 band p15.5, have been identified using Southern blotting experiments. This polymorphism can be easily assayed by polymerase chain reaction (PCR) to detect linkage of inherited disorder. Five alleles were observed in 86 unrelated individuals due to 3–8 direct perfect repeats of 59 bp. This repeat has the particularity to begin at the end of the preceding exon. Southern blot experiments revealed the locus specificity of the repeat. The sequence of the repeat unit does not match the consensus sequence of Chi‐related minisatellites.