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DNA profiling of orangutan ( Pongo pygmaeus ) feces to prove descent and identity in wildlife animals
Author(s) -
Immel UtaDorothee,
Hummel Susanne,
Herrmann Bernd
Publication year - 1999
Publication title -
electrophoresis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.666
H-Index - 158
eISSN - 1522-2683
pISSN - 0173-0835
DOI - 10.1002/(sici)1522-2683(19990101)20:8<1768::aid-elps1768>3.0.co;2-y
Subject(s) - pongo pygmaeus , biology , kinship , dna profiling , str analysis , genetics , amelogenin , microsatellite , evolutionary biology , identity by descent , polymerase chain reaction , dna , allele , zoology , gene , haplotype , political science , law
In this study, orangutan ( Pongo pygmaeus ssp.) DNA was extracted from excrements, obtained from individual zoo populations. To examine whether human short tandem repeat loci (STR) are suitable for the reconstruction of kinship in orangutans, nine STRs, commonly used in forensic studies in man and the amelogenin system, were amplified in a multiplex polymerase chain reaction (PCR) approach. Five of the nine human autosomal STRs in question amplified successfully in orangutans. Furthermore, the experiments revealed that orangutans possess allele distributions comparable to humans in these systems. Fragment length determination allowed kinship structures to be reconstructed.