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Ancient DNA profiling by megaplex amplications
Author(s) -
Hummel Susanne,
Schultes Tobias,
Bramanti Barbara,
Herrmann Bernd
Publication year - 1999
Publication title -
electrophoresis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.666
H-Index - 158
eISSN - 1522-2683
pISSN - 0173-0835
DOI - 10.1002/(sici)1522-2683(19990101)20:8<1717::aid-elps1717>3.0.co;2-d
Subject(s) - ancient dna , dna profiling , profiling (computer programming) , computational biology , dna , biology , evolutionary biology , genetics , computer science , medicine , population , environmental health , operating system
Simultaneous amplification of nine human short tandem repeat (STR) DNA sequences and the amelogenin locus allows reducing to an absolute minimum the amount of sample material that is necessary for genetic identification or kinship analysis. Valuable remains can be studied this way without any visible damage, as is demonstrated by typing the DNA of a tooth root from the Saxon warrior Widukind, who died about 1200 years ago. The broad applicability of the megaplex approach is shown by typing bone and teeth specimens ranging from a few months to 3000 years of age employing AmpFl STR Profiler Plus. Additionally, megaplex STR typing is the method of choice for proving the authenticity of molecular results derived from ancient degraded DNA.