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Microplate array diagonal gel electrophoresis for mutation research in DNA banks
Author(s) -
Day Ian N. M.,
Spanakis Emmanuel,
Chen Xiaohe,
O'Dell Sandra D.
Publication year - 1999
Publication title -
electrophoresis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.666
H-Index - 158
eISSN - 1522-2683
pISSN - 0173-0835
DOI - 10.1002/(sici)1522-2683(19990101)20:6<1250::aid-elps1250>3.0.co;2-5
Subject(s) - gel electrophoresis , computational biology , biology , polyacrylamide gel electrophoresis , genetics , microbiology and biotechnology , biochemistry , enzyme
Molecular genetic epidemiology, association and linkage studies in populations, human or other species, is now yielding powerful new insights into disease and susceptibility genes. Inter alia , the subject requires laboratory analytical methodologies focused on achieving high throughput. Here we review one suite of methodology suitable for such laboratories. Microplate array diagonal gel electrophoresis (MADGE) was invented for molecular genetic epidemiological studies. It combines direct compatibility with microplates, convenient polyacrylamide gel electrophoresis and economy of time and reagents, at minimal capital cost, and enables one user to run up to several thousand gel lanes per day for direct assay of single‐base variations. Melt‐MADGE combines temporal thermal ramp apparatus to achieve similar throughput for de novo mutation scanning.