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Characterisation of stem cell expression using two‐dimensional electrophoresis
Author(s) -
Pearce Alison,
Svendsen Clive N.
Publication year - 1999
Publication title -
electrophoresis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.666
H-Index - 158
eISSN - 1522-2683
pISSN - 0173-0835
DOI - 10.1002/(sici)1522-2683(19990101)20:4/5<969::aid-elps969>3.0.co;2-f
Subject(s) - stem cell , fibroblast growth factor , biology , microbiology and biotechnology , cellular differentiation , cell , electrophoresis , fibroblast , lysis , gel electrophoresis , cell culture , chemistry , biochemistry , genetics , gene , receptor
Stem cells were isolated from foetal human brain tissue and induced to proliferate using the mitogens epidermal growth factor (EFG) and fibroblast growth factor (FGF). Under these conditions the dividing cells formed spheres which could be maintained in an undifferentiated state for extended periods of time. Following removal of the mitogen and addition of serum, the human stem cells rapidly began to differentiate. Samples from differentiated and undifferentiated cultures were lysed and analysed using two‐dimensional (2‐D) electrophoresis, a powerful technique for the separation and characterisation of proteins in complex mixtures. After 1 h post‐differentiation, marked differences in protein expression could be observed between undifferentiated and differentiated stem cell samples.