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Proteome analysis of factor for inversion stimulation (Fis) overproduction in Escherichia coli
Author(s) -
Choe Leila H.,
Chen Wilfred,
Lee Kelvin H.
Publication year - 1999
Publication title -
electrophoresis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.666
H-Index - 158
eISSN - 1522-2683
pISSN - 0173-0835
DOI - 10.1002/(sici)1522-2683(19990101)20:4/5<798::aid-elps798>3.0.co;2-f
Subject(s) - periplasmic space , proteome , ribosomal protein , escherichia coli , ribosomal rna , transcription factor , elongation factor , microbiology and biotechnology , biology , transcription (linguistics) , rna , chemistry , biochemistry , gene , ribosome , linguistics , philosophy
The factor‐for‐inversion stimulation protein (Fis) is a global regulatory protein in Escherichia coli that activates ribosomal RNA (rRNA) transcription by binding to three upstream activation sites of the rRNA promoter and enhances transcription 5‐ to 10‐fold in vivo . Fis overexpression results in different effects on cell growth depending on nutrient conditions. Differential proteome analysis of Fis‐expressing cells shows ten protein spots corresponding to Fis overexpression in both rich (YT) and minimal (M9+glucose) media. Three of these spots have been identified as elongation factor TS, histidine‐binding periplasmic protein precursor, and ketol‐acid reductoisomerase.