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Tryptic digest mapping by gradient capillary electrochromatography
Author(s) -
Behnke Beate,
Metzger Jörg W.
Publication year - 1999
Publication title -
electrophoresis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.666
H-Index - 158
eISSN - 1522-2683
pISSN - 0173-0835
DOI - 10.1002/(sici)1522-2683(19990101)20:1<80::aid-elps80>3.0.co;2-i
Subject(s) - capillary electrochromatography , chromatography , capillary action , electrochromatography , chemistry , joule heating , high performance liquid chromatography , resolution (logic) , analytical chemistry (journal) , capillary electrophoresis , materials science , artificial intelligence , computer science , composite material
A high performance liquid chromatography (HPLC) system complemented with T‐split, capillary detection cell and a high voltage power supply was used for peptide mapping by gradient electrochromatography and nanoliquid chromatography (nano‐LC). With capillary columns of 100 μm ID, 6 cm packed with octadecylated 1.5 μm silica particles, the typical analysis time was approximately 10–15 min. The resolution of a tryptic digest of cytochrome c obtained by electrochromatography at 100 kV/m was superior compared to the analysis by nano‐LC. Bubble formation caused by Joule heating at currents up to 100 μA was successfully suppressed by using a resistor capillary of 25 μm ID connected to the outlet of the packed column.