Premium
Silicate entrapped columns — new columns designed for capillary electrochromatography
Author(s) -
Chirica Gabriela,
Remcho Vincent T.
Publication year - 1999
Publication title -
electrophoresis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.666
H-Index - 158
eISSN - 1522-2683
pISSN - 0173-0835
DOI - 10.1002/(sici)1522-2683(19990101)20:1<50::aid-elps50>3.0.co;2-o
Subject(s) - capillary electrochromatography , capillary action , monolith , chromatography , electrochromatography , packed bed , silicate , breakage , materials science , electro osmosis , chemical engineering , porosity , chemistry , composite material , electrophoresis , catalysis , organic chemistry , engineering
Designed especially for capillary electrochromatography (CEC), silicate‐entrapped columns are made by trapping particles of chromatographic packing material in a network of silica. Once entrapped, the capillary no longer requires frits. This renders a more homogeneous and stable packed bed. Accidental breakage of the fragile frits is not an issue with these robust columns. Columns packed with reverse‐phase material subjected to silicate entrapment demonstrated faster separations of retained analytes and increased efficiencies compared with nonentrapped columns. The method was also used to prepare chiral CEC columns by entrapping a molecular imprinted polymeric (MIP) packing having minimal surface charge density, thus being unable alone to support sufficient electroosmotic flow for CEC.