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Simultaneous separation and enantioresolution of racemic local anesthetic drugs by capillary zone electrophoresis with Tween 20 and methyl‐β‐cyclodextrin as selectors, employing a double plug technique
Author(s) -
Amini Ahmad,
Jäverfalk Emmy,
Bastami Salumeh,
Westerlund Douglas
Publication year - 1999
Publication title -
electrophoresis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.666
H-Index - 158
eISSN - 1522-2683
pISSN - 0173-0835
DOI - 10.1002/(sici)1522-2683(19990101)20:1<204::aid-elps204>3.0.co;2-i
Subject(s) - enantiomer , capillary electrophoresis , chromatography , chemistry , cyclodextrin , capillary action , spark plug , micellar electrokinetic chromatography , electrophoresis , phase (matter) , micelle , resolution (logic) , analytical chemistry (journal) , materials science , stereochemistry , organic chemistry , aqueous solution , engineering , composite material , aerospace engineering , artificial intelligence , computer science
A new approach for simultaneous chiral and achiral separations by capillary zone electrophoresis is described. Two adjacent selector plugs, consisting of Tween 20 as an achiral and methyl‐β‐cyclodextrin (CD) as a chiral selector, are employed and four related local anesthetics are used as model compounds. The principles of the partial filling technique, whereby the capillary is filled with the chiral selector solution followed by the micellar solution at different plug lengths and concentrations, prior to application of the solutes, was employed. During the run both capillary ends were dipped in a simple buffer, i.e. , one without additives. The two separation media worked independently without any interaction. Separation of the solutes and their enantiomers was regulated by adjusting both the concentration and plug length (PL) of the micellar solution in the capillary, employing methyl β‐CD as chiral selector either at 38 or 76 m M . The solutes were separated on the basis of their affinity towards the micellar phase before they reached the methyl‐β‐CD plug for enantioseparation. In the absence of the micellar plug, the enantiomers of prilocaine overlapped those of bupivacaine. The solutes and their enantiomers were completely separated by employing two adjacent plugs consisting of 100 m M Tween 20 solution (PL ≈ 10 cm) and methyl‐β‐CD solution at either 38 or 76 m M (PL ≈ 30 cm).