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Highly efficient separation of isomeric epoxy fatty acids by micellar electrokinetic chromatography
Author(s) -
Wan Hong,
Blomberg Lars G.,
Hamberg Mats
Publication year - 1999
Publication title -
electrophoresis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.666
H-Index - 158
eISSN - 1522-2683
pISSN - 0173-0835
DOI - 10.1002/(sici)1522-2683(19990101)20:1<132::aid-elps132>3.0.co;2-i
Subject(s) - micellar electrokinetic chromatography , chemistry , chromatography , sodium dodecyl sulfate , capillary electrophoresis , micelle , acetonitrile , factorial experiment , electrolyte , theoretical plate , epoxy , electrokinetic phenomena , organic chemistry , aqueous solution , statistics , mathematics , electrode
A capillary electrophoresis (CE) method has been developed for simple and direct separation of cis ‐ and trans ‐12,13‐epoxy‐9( Z )‐octadecenoic acid and 9,10‐epoxy‐12( Z )‐octadecenoic acid isomers. Separation was performed in micellar electrokinetic capillary chromatography (MEKC) using a buffer consisting of 25 m M borate (pH 9.20), 10 m M sodium dodecyl sulfate (SDS) and 10% v/v acetonitrile. The key variables, concentrations of SDS and organic modifier, were optimized by the application of a factorial experimental design. The use of a low micellar concentration, just above critical micelle concentration (CMC), in a background electrolyte containing an organic modifier not only made it possible to dissolve and separate highly hydrophobic fatty acid isomers, but also resulted in improved separation efficiency and selectivity. Separation efficiency up to 4 × 10 5 theoretical plates/m was achieved under an optimized condition. Also investigated were the influence of temperature on separation and the effect of organic modifier concentration on the dynamic exchange of the analytes between micelles and the bulk of the buffer solution. Direct UV was applied for detection of the fatty acids.