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Microchannel electrophoretic separation of biogenic amines by micellar electrokinetic chromatography
Author(s) -
Rodríguez Isabel,
Lee Hian Kee,
Li Sam F. Y.
Publication year - 1999
Publication title -
electrophoresis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.666
H-Index - 158
eISSN - 1522-2683
pISSN - 0173-0835
DOI - 10.1002/(sici)1522-2683(19990101)20:1<118::aid-elps118>3.0.co;2-2
Subject(s) - chromatography , cadaverine , chemistry , micellar electrokinetic chromatography , capillary electrophoresis , spermidine , electrophoresis , tryptamine , biogenic amine , putrescine , pyrilamine , amine gas treating , spermine , organic chemistry , biochemistry , receptor , neurotransmitter , antagonist , enzyme
Abstract Fast, efficient separation of most common biogenic amines was successfully performed on a glass microchip capillary electrophoresis device. The amines putrescine, histamine, tyramine, cadaverine, phenethylamine, tryptamine, spermidine and spermine were derivatized prior to fluorescence detection with fluorescein isothiocyanate. Separation was carried out using a channel length of 28 mm, a cross section of 50 × 8 μm, and a field strength of 600 V/cm. After optimization of buffer electrolyte conditions (120 m M boric acid, pH 9.4, modified with 40 m M SDS), fluorescein thiocarbamyl amine derivatives were successfully resolved. Analysis time was as short as 75 s. Determination of the biogenic amines was achieved in soy sauce samples.