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High‐Loading Supports for Oligonucleotide Synthesis
Author(s) -
Patnaik Anupa K.,
Subba Rao N.,
Kumar Pradeep,
Sharma Ashwani K.,
Garg Bhagwan S.,
Gupta Kailash C.
Publication year - 2000
Publication title -
helvetica chimica acta
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.74
H-Index - 82
eISSN - 1522-2675
pISSN - 0018-019X
DOI - 10.1002/(sici)1522-2675(20000216)83:2<322::aid-hlca322>3.0.co;2-a
Subject(s) - chemistry , oligonucleotide , combinatorial chemistry , oligonucleotide synthesis , reagent , dna , organic chemistry , biochemistry
A simple protocol for the preparation of high‐loading supports, suitable for large‐scale synthesis of oligonucleotides, has been developed. The method involves the use of inexpensive reagents and is amenable to large‐scale preparation of supports. The derivatized supports were successfully employed in an automated DNA synthesizer without any difficulty. The quality of the synthesized oligonucleotides was found to be comparable to that of the corresponding oligomers prepared with commercially available standard supports.