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Nucleotides, Part LXI , Phthaloyl Strategy: A New Concept of Oligonucleotide Synthesis
Author(s) -
Beier Markus,
Pfleiderer Wolfgang
Publication year - 1999
Publication title -
helvetica chimica acta
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.74
H-Index - 82
eISSN - 1522-2675
pISSN - 0018-019X
DOI - 10.1002/(sici)1522-2675(19990407)82:4<633::aid-hlca633>3.0.co;2-u
Subject(s) - chemistry , oligonucleotide , nucleotide , oligonucleotide synthesis , combinatorial chemistry , computational biology , biochemistry , dna , gene , biology
A new alternative strategy of oligonucleotide synthesis was developed by use of the phthaloyl protecting group for the exocyclic amino functions of the nucleobases (see 9 – 12 ). This approach combines the advantages of cheap and easily accessable monomeric building blocks (see 17 – 20 ), standard machine‐aided oligonucleotide synthesis, and a fast deprotection protocol which is orthogonal to the cleavage procedure from the solid support. The crude oligonucleotides show high purity and require, in general, no further chromatographic purification.