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3D localized 1 H‐ 13 C heteronuclear single‐quantum coherence correlation spectroscopy in vivo
Author(s) -
Watanabe H.,
Ishihara Y.,
Okamoto K.,
Oshio K.,
Kanamatsu T.,
Tsukada Y.
Publication year - 2000
Publication title -
magnetic resonance in medicine
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.696
H-Index - 225
eISSN - 1522-2594
pISSN - 0740-3194
DOI - 10.1002/(sici)1522-2594(200002)43:2<200::aid-mrm6>3.0.co;2-h
Subject(s) - heteronuclear molecule , coherence (philosophical gambling strategy) , nuclear magnetic resonance , spectroscopy , heteronuclear single quantum coherence spectroscopy , nuclear magnetic resonance spectroscopy , two dimensional nuclear magnetic resonance spectroscopy , physics , chemistry , materials science , quantum mechanics
A method for spatially three‐dimensional (3D) localized two‐dimensional (2D) 1 H‐ 13 C correlation spectroscopy, localized HSQC, is proposed. This method has the following special feature in the preparation period. The 180°( 13 C) and 180°( 1 H) pulses are separated in time, and the 180°( 13 C) pulse is applied at 1/(4 1 J CH ) before the 90°( 1 H) polarization transfer pulse. The preparation (echo) period 2τ can then be set substantially longer than 1/(2 1 J CH ), so that even in a whole‐body system, slice‐selective 90°( 1 H) pulses and gradient pulses can be applied in that period. The localization capabilities of this method were confirmed in a phantom experiment. The 3D localized 2D 1 H‐ 13 C correlation spectra from a monkey brain in vivo were obtained after [1‐ 13 C]glucose injection, and amino acid metabolism was detected; that is, [4‐ 13 C]glutamate appeared immediately after the injection, followed by the appearance of [2‐ 13 C]glutamate, [3‐ 13 C]glutamate, and [4‐ 13 C]glutamine. Magn Reson Med 43:200–210, 2000. © 2000 Wiley‐Liss, Inc.