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MRI measurement of cell volume fraction in the perfused rat hippocampal slice
Author(s) -
Buckley David L.,
Bui Jonathan D.,
Phillips M. Ian,
Blackband Stephen J.
Publication year - 1999
Publication title -
magnetic resonance in medicine
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.696
H-Index - 225
eISSN - 1522-2594
pISSN - 0740-3194
DOI - 10.1002/(sici)1522-2594(199909)42:3<603::aid-mrm25>3.0.co;2-q
Subject(s) - perfusion , slice preparation , volume fraction , volume (thermodynamics) , fraction (chemistry) , nuclear medicine , chemistry , nuclear magnetic resonance , biomedical engineering , chromatography , medicine , physics , radiology , in vitro , quantum mechanics , biochemistry
T 1 ‐weighted NMR imaging of the isolated perfused rat hippo‐campal slice was used to estimate cell volume fraction. Eight brain slices were studied in artificial cerebrospinal fluid (aCSF) using a 600 MHz narrow bore spectrometer and a home built perfusion chamber. Cell volume fraction was calculated as 1 − f ECS , where f ECS is the distribution volume of gadodiamide in the slice. This was determined by measuring the T 1 of the slice before and after perfusion with gadodiamde. A mean cell volume fraction of 0.66 ± 0.04 was estimated. The addition of 60 mM mannitol to three of the brain slices produced a 26% decrease in the cell volume fraction. The technique affords a simple means of estimating cell volume fraction and can be extended to produce images reflecting cell density. Magn Reson Med 42:603–607, 1999. © 1999 Wiley‐Liss, Inc.