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Multiple bond 13 C‐ 13 C spin‐spin coupling provides complementary information in a 13 C NMR isotopomer analysis of glutamate
Author(s) -
Carvalho Rui A.,
Babcock Evelyn E.,
Jeffrey F. Mark H.,
Sherry A. Dean,
Malloy Craig R.
Publication year - 1999
Publication title -
magnetic resonance in medicine
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.696
H-Index - 225
eISSN - 1522-2594
pISSN - 0740-3194
DOI - 10.1002/(sici)1522-2594(199907)42:1<197::aid-mrm26>3.0.co;2-5
Subject(s) - isotopomers , chemistry , carbon 13 nmr , nuclear magnetic resonance , citric acid cycle , propionate , stereochemistry , enzyme , biochemistry , molecule , physics , organic chemistry
Most 13 C nuclear magnetic resonance (NMR) isotopomer analyses relate a metabolic index of interest to populations of 13 C isotopomers as reported by one‐bond 13 C‐ 13 C spin‐spin couplings. Metabolic conditions that produce highly enriched citric acid cycle intermediates often lead to 13 C NMR spectra of metabolites such as glutamate that show extra multiplets due to long‐range couplings. It can be demonstrated from 13 C NMR spectra of hearts perfused with mixtures of acetate plus propionate that multiplets in glutamate C2 arising from 3 J 25 coupling provide a direct readout of acetyl‐CoA fractional enrichment (F C1 and F C3 ), while multiplets in glutamate C5 arising from 2 J 35 and 3 J 25 couplings quantitatively reflect enrichment of the anaplerotic substrate. Magn Reson Med 42:197–200, 1999. © 1999 Wiley‐Liss, Inc.