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In vivo observation of lactate methyl proton magnetization transfer in rat C6 glioma
Author(s) -
Luo Yanping,
Rydzewski Jan,
de Graaf Robin A.,
Gruetter Rolf,
Garwood Michael,
Schleich Thomas
Publication year - 1999
Publication title -
magnetic resonance in medicine
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.696
H-Index - 225
eISSN - 1522-2594
pISSN - 0740-3194
DOI - 10.1002/(sici)1522-2594(199904)41:4<676::aid-mrm5>3.0.co;2-d
Subject(s) - magnetization transfer , chemistry , magnetization , nuclear magnetic resonance , in vivo , nuclear magnetic resonance spectroscopy , analytical chemistry (journal) , magnetic resonance imaging , magnetic field , physics , chromatography , biology , medicine , microbiology and biotechnology , quantum mechanics , radiology
Magnetic resonance spectroscopy (MRS) measurements of the lactate methyl proton in rat brain C6 glioma tissue acquired in the presence of an off‐resonance irradiation field, analyzed using coupled Bloch equation formalism assuming two spin pools, demonstrated the occurrence of magnetization transfer. Quantitative analysis revealed that a very small fraction of lactate ( f = 0.0012) is rotationally immobilized despite a large magnetization transfer effect. Off‐resonance rotating frame spin‐lattice relaxation studies demonstrated that deuterated lactate binds to bovine serum albumin and the proteins present in human plasma, thereby providing a possible physical basis for the observed magnetization transfer effect. These results demonstrate that partial or complete saturation of the motionally restricted lactate pool (as well as other metabolites) by the application of an off‐resonance irradiation field, such as that used for water presaturation, can lead to a substantial decrease in resonance intensity by way of magnetization transfer effects, resulting in quantitation errors. Magn Reson Med 41:676–685, 1999. © 1999 Wiley‐Liss, Inc.