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Concentrations of human cardiac phosphorus metabolites determined by SLOOP 31 P NMR spectroscopy
Author(s) -
Meininger Martin,
Landschütz Wilfried,
Beer Meinrad,
Seyfarth Tobias,
Horn Michael,
Pabst Thomas,
Haase Axel,
Hahn Dietbert,
Neubauer Stefan,
von Kienlin Markus
Publication year - 1999
Publication title -
magnetic resonance in medicine
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.696
H-Index - 225
eISSN - 1522-2594
pISSN - 0740-3194
DOI - 10.1002/(sici)1522-2594(199904)41:4<657::aid-mrm3>3.0.co;2-i
Subject(s) - nuclear magnetic resonance spectroscopy , phosphorus , nuclear magnetic resonance , chemistry , phosphorus 31 nmr spectroscopy , spectroscopy , stereochemistry , organic chemistry , physics , quantum mechanics
Human cardiac 31 P nuclear magnetic resonance (NMR) spectra are usually quantified in relative terms, i.e., the ratio of metabolite signals is calculated. If 31 P NMR spectroscopy of the heart is to emerge as a clinically relevant diagnostic modality, reliable quantification of absolute concentrations of 31 P metabolites is required. We applied spectral localization with optimal point spread function (SLOOP) 31 P NMR spectroscopy to measure absolute concentrations of phosphocreatine (PCr) and adenosine triphosphate (ATP) in human myocardium. The accuracy of the quantification was first validated in a phantom study. Seven healthy volunteers (aged 19–29 years) were then examined at 1.5 T using a nominal spatial resolution of 25 mL. SLOOP allowed us to obtain localized spectra from compartments anatomically matched to the left ventricular wall. The a priori knowledge of the anatomical structure was obtained from 1 H images. The spatially varying effects of saturation, off‐resonance, and sensitivity were considered during the reconstruction process. Metabolites were quantified with reference to an external 31 P standard. Concentrations of 9.0 ± 1.2 and 5.3 ± 1.2 mmol/kg wet wt (mean ± SD, n = 9) were determined for PCr and ATP in normal heart, respectively. The influence of nuclear Overhauser enhancement on metabolite quantification is discussed. Magn Reson Med 41:657–663, 1999. © 1999 Wiley‐Liss, Inc.