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Long‐term effects of repetitive exposure to a static magnetic field (1.5 T) on proliferation of human fetal lung fibroblasts
Author(s) -
Wiskirchen J.,
Groenewaeller E.F.,
Kehlbach R.,
Heinzelmann F.,
Wittau M.,
Rodemann H.P.,
Claussen C.D.,
Duda S.H.
Publication year - 1999
Publication title -
magnetic resonance in medicine
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.696
H-Index - 225
eISSN - 1522-2594
pISSN - 0740-3194
DOI - 10.1002/(sici)1522-2594(199903)41:3<464::aid-mrm6>3.0.co;2-r
Subject(s) - clonogenic assay , fibroblast , population , cell growth , bromodeoxyuridine , fetus , andrology , dna synthesis , cell cycle , biology , lung , immunology , microbiology and biotechnology , cell , chemistry , cell culture , medicine , dna , biochemistry , genetics , pregnancy , environmental health
The aim of the study was to assess the effects of repetitive exposures to a static magnetic field (1.5 T) on human fetal lung fibroblast (HFL) proliferation. HFL were exposed three times a week for 1 hr to a static magnetic field for 3 weeks. Cells were subcultured every week. Population doublings (PD) and cumulative population doublings (CPD) were calculated weekly. Colony formation assays, bromodeoxyuridine enzyme‐linked immunosorbent assay, and cell cycle analysis were performed weekly. After the third week, proliferation kinetics were assessed. Over a period of 3 weeks no statistically significant differences between the PD and CPD of exposed and control cells could be detected. Clonogenic activity, DNA synthesis, cell cycle, and proliferation kinetics were not altered by magnetic field exposure. The data do not provide evidence that repetitive exposures to a static magnetic field (1.5 T) exert effects on HFL proliferation. Magn Reson Med 41:464–468, 1999. © 1999 Wiley‐Liss, Inc.