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Determination of a Hair Dye Using Different Derivatization Agents and HPLC in Combination with UV, Electrochemical, and Fluorescence Detection
Author(s) -
Goger Birgit,
Wintersteiger Reinhold,
Beck Heinz
Publication year - 1999
Publication title -
archiv der pharmazie
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.468
H-Index - 61
eISSN - 1521-4184
pISSN - 0365-6233
DOI - 10.1002/(sici)1521-4184(199911)332:11<399::aid-ardp399>3.0.co;2-q
Subject(s) - derivatization , chemistry , chromatography , fluorescence , selectivity , coulometry , high performance liquid chromatography , hair dyes , electrochemistry , organic chemistry , electrode , physics , quantum mechanics , dyeing , catalysis
Comparative studies using UV, fluorescence, and electrochemical detection after HPLC separation were performed to determine the hair dye N,N ‐bis(hydroxyethyl)‐ p ‐phenylenediamine PPDA . For the analysis of this substance in the extraction fluid with regard to skin permeation, sensitivity and selectivity were the most important parameters. Reaction with 2‐(4‐isocyanatophenyl)‐6‐methylbenzothiazole MBP and subsequent coulometric detection in the oxidative mode proved to be superior to fluorescence and UV detection. As future hair dyes may also possess amino and/or hydroxyl groups the method developed may be transferable.