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Biotransformation and Toxicity of the Lipoxygenase Inhibitor 2‐Hydroxy‐5‐methyllaurophenone Oxime (FLM 5011) on Hep G2 Cells
Author(s) -
Mühlenfeld Katrin,
Langner Andreas
Publication year - 1998
Publication title -
archiv der pharmazie
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.468
H-Index - 61
eISSN - 1521-4184
pISSN - 0365-6233
DOI - 10.1002/(sici)1521-4184(199807)331:7/8<259::aid-ardp259>3.0.co;2-7
Subject(s) - hep g2 , biotransformation , metabolite , toxicity , chemistry , lipoxygenase , cell culture , cytotoxicity , apoptosis , oxime , hepatocyte , urine , arachidonate 5 lipoxygenase , pharmacology , cell growth , biochemistry , enzyme , in vitro , biology , arachidonic acid , organic chemistry , genetics
2‐Hydroxy‐5‐methyllaurophenone oxime (FLM 5011) is an inhibitor of lipoxygenase with antiinflammatory and antiallergic actions. We incubated FLM 5011 on the human immortal cell line Hep G2 and found a similar metabolite spectrum as in rat urine and faeces. We also measured the cytotoxicity of FLM 5011 on Hep G2 monolayers by the amido black assay and found that the influence of cell proliferation is partly due to apoptotic activities. Although the metabolic activity of Hep G2 cells is lower compared to rat hepatocyte cultures they are a suitable test system for biotransformation studies. Their higher proliferation rate allows toxicity to be characterised more exactly.