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Determination of Propranolol by Capillary Electrophoresis with End‐Column Amperometric Detection
Author(s) -
Bai Xiaoxia,
You Tianyan,
Sun Hanwen,
Yang Xiurong,
Wang Erkang
Publication year - 2000
Publication title -
electroanalysis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.574
H-Index - 128
eISSN - 1521-4109
pISSN - 1040-0397
DOI - 10.1002/(sici)1521-4109(200005)12:7<535::aid-elan535>3.0.co;2-y
Subject(s) - amperometry , capillary electrophoresis , detection limit , chromatography , chemistry , analytical chemistry (journal) , capillary action , electrode , electrophoresis , phosphate buffered saline , materials science , electrochemistry , composite material
A capillary electrophoresis‐amperometric detection system was developed for the determination of propranolol (PRO) at a 33 µm carbon fiber microdisk electrode (CFE). The cyclic voltammogram, the hydrodynamic voltammograms and the effect of pH were studied. Under the optimum conditions: separation voltage 15 kV; injection 3 s at 15 kV; 10 mM pH 7.5 phosphate buffer, 1.15 V (vs. Ag/AgCl) detection potential, the detection limit (LOD) for PRO was 0.05 µM ( S/N =3). The response for PRO was linear over two orders of magnitude with a linear correlation coefficient of 0.994. The feasibility of this method was demonstrated by the detection of PRO in urine sample.