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A New Solid‐State pH Sensor and Its Application in the Construction of all Solid‐State Urea Biosensor
Author(s) -
Pandey P. C.,
Upadhyay S.,
Singh G.,
Prakash R.,
Srivastava R. C.,
Seth P. K.
Publication year - 2000
Publication title -
electroanalysis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.574
H-Index - 128
eISSN - 1521-4109
pISSN - 1040-0397
DOI - 10.1002/(sici)1521-4109(200005)12:7<517::aid-elan517>3.0.co;2-y
Subject(s) - urease , urea , polymer , biosensor , electrode , chemistry , vinyl chloride , inorganic chemistry , matrix (chemical analysis) , adsorption , reference electrode , analytical chemistry (journal) , materials science , electrochemistry , chromatography , nanotechnology , organic chemistry , copolymer
A new solid‐state pH sensor is developed using neutral poly(3‐cyclohexyl thiophene) assembled over a Pt disk electrode. The new sensor is developed following two different approaches; 1) the neutral poly(3‐cyclohexyl thiophene) dissolved in chloroform and subsequent coating on to a Pt disk electrode; 2) the neutral polymer is incorporated into plasticized poly(vinyl chloride) matrix membrane. In both cases the polymer modified electrode is sensitive to pH and a reversible super Nernstian behavior is observed. The typical response of the pH sensor and its reversibility are reported. The polymer coated electrode is subsequently used to construct an all solid‐state urea sensor. The construction of this new urea sensor involves the following two major steps; a) 20 µL of urease solution (40 mg /mL) is allowed to assemble overnight at 4 °C over neutral poly (3‐cyclohexyl thiophene) modified electrode; b) an organically modified sol‐gel layer is allowed to form over the urease adsorbed polymer modified electrode. The new solid‐state urea sensor provides excellent reproducibility of the measurements and is stable for 3 months when stored at 4 °C under dry condition. The typical response of the solid‐state urea sensor and the calibration plot of urea analysis are reported.