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The Computer Aided Design and Experimental Development of a New Device for the Measurement of Electrochemiluminescence
Author(s) -
Fulian Q.,
Fisher A. C.,
Riley D. J.
Publication year - 2000
Publication title -
electroanalysis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.574
H-Index - 128
eISSN - 1521-4109
pISSN - 1040-0397
DOI - 10.1002/(sici)1521-4109(200005)12:7<503::aid-elan503>3.0.co;2-h
Subject(s) - electrode , electrochemiluminescence , volumetric flow rate , range (aeronautics) , flow (mathematics) , analytical chemistry (journal) , intensity (physics) , sensitivity (control systems) , volume (thermodynamics) , channel (broadcasting) , materials science , biological system , chemistry , computer science , mechanics , electronic engineering , optics , physics , chromatography , thermodynamics , engineering , computer network , biology , composite material
A new hydrodynamic technique is presented which provides quantitative spatial resolution of electrogenerated chemiluminescene (ECL) under steady state voltammetric operating conditions. Specifically a channel flow cell arrangement was employed exploiting a double electrode configuration. The two electrodes were utilized to electrogenerate different reactants separately and the solution velocity exploited to bring the generated reactants together. The ECL spectra were recorded using a charge coupled device (CCD) which permits the full spectrum to be measured in intervals of approximately 10 ms. Alternatively the CCD permits the variation of intensity to be recorded as a function of the cell position and this was also exploited within these investigations. The cell configuration and volume flow rate range were optimized for maximum ECL detection sensitivity using a computer aided design strategy. This was achieved by the use of a finite difference model which predicts the concentration profiles within the channel cell for a range of electrode arrangements. The validity of the new strategy is evaluated by experimental studies utilizing the well documented 9,10‐diphenylanthracene (DPA) system. The variation of ECL intensity as a function of the volume flow rate permits kinetic and mechanistic data to be derived via comparison with appropriate numerical modeling for candidate mechanisms. Good agreement is obtained between theory and experimental measurements.