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Electrochemical Detection of Secreted Alkaline Phosphatase: Implications to Cell Based Assays
Author(s) -
Kelso Elizabeth,
McLean John,
Cardosi Marco F.
Publication year - 2000
Publication title -
electroanalysis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.574
H-Index - 128
eISSN - 1521-4109
pISSN - 1040-0397
DOI - 10.1002/(sici)1521-4109(200005)12:7<490::aid-elan490>3.0.co;2-y
Subject(s) - alkaline phosphatase , electrochemistry , substrate (aquarium) , chemistry , herpes simplex virus , electrode , detection limit , cyclic voltammetry , enzyme , cell , placental alkaline phosphatase , biochemistry , chromatography , biology , virus , virology , ecology
A sensitive electrochemical assay method is described for secreted placental alkaline phosphatase (SPAP). The enzyme activity is measured using 2‐naphthyl phosphate as the electrochemical substrate. The product of the reaction, 2‐naphthol, is detected using Osteryoung square wave voltammetry (OSWV) with good sensitivity and precision using in‐house screen printed carbon electrode assembly. The assay format is adapted to develop a cell based assay for herpes simplex virus (HSV).