Premium
Determination of Buprenorphine in Pharmaceuticals and Human Urine by Adsorptive Stripping Voltammetry in Batch and Flow Systems
Author(s) -
GarcíaFernández M. Angeles,
FernándezAbedul M. Teresa,
CostaGarcía Agustín
Publication year - 2000
Publication title -
electroanalysis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.574
H-Index - 128
eISSN - 1521-4109
pISSN - 1040-0397
DOI - 10.1002/(sici)1521-4109(200005)12:7<483::aid-elan483>3.0.co;2-q
Subject(s) - buprenorphine , chromatography , adsorptive stripping voltammetry , differential pulse voltammetry , chemistry , reproducibility , adsorption , urine , cyclic voltammetry , flow injection analysis , electrode , voltammetry , detection limit , electrochemistry , organic chemistry , biochemistry , receptor , opioid
The adsorption of buprenorphine on carbon paste electrodes has been studied by cyclic (CV) and differential pulse voltammetry (DPV). By applying a simple electrode pretreatment good reproducibility of the current signal is obtained (RSD=0.85 %, n =6 in CV and RSD=0. 35 %, n =6 in DPV for a 5.0×10 –7 M buprenorphine concentration in BR buffer pH 9.0). These methods permit a very sensitive (LD=2.0×10 –8 M) and selective determination. They were applied to the quantitation of buprenorphine in human urine coming from subjects following buprenorphine treatment. The methodology was coupled to a flow system incorporating a thin‐layer flow cell. A linear response was obtained in the concentration range comprised between 1.0×10 –8 and 8.0×10 –7 M. The application to the determination of this molecule in pharmaceuticals (vials and tablets) and human urine without any sample pretreatment is studied.